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Ribosomes: Analysis by Cesium Sulfate Gradient Centrifugation

F M DeFilippes

Science. 1965 Oct 29;150(3696):610-2.

PMID: 5837097

Abstract:

Ribosomes from the cytoplasm of a mammalian tissue, HeLa cells, were partially purified and then concentrated into a zone by prolonged centrifugation in Cs(2)SO(4) solution (density, 1.43 grams per milliliter). Ribosomal preparations treated with bentonite prior to density-gradient centrifugation showed single peaks after centrifugation. Material recovered from the peaks represented aggregates of the original 74S particle.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP10294549 Cesium sulfate Cesium sulfate 10294-54-9 Price
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