0

Role of Lipoxygenase in Xenobiotic Metabolism: Sulfoxidation of Thiobenzamide by Purified Soybean Lipoxygenase

A K Naidu, A P Kulkarni

Res Commun Chem Pathol Pharmacol. 1991 Feb;71(2):175-88.

PMID: 1904618

Abstract:

Earlier this laboratory recognized lipoxygenase catalyzed reactions as a novel pathway for xenobiotic metabolism. To further explore the spectrum of reactions catalyzed by lipoxygenase, sulfoxidation of thiobenzamide was studied. Purified soybean lipoxygenase was found to oxidize thiobenzamide to thiobenzamide sulfoxide in the presence of linoleic acid at a rate of 241 nmoles/min/nmole enzyme. The reaction was dependent upon enzyme, pH, thiobenzamide and linoleic acid concentration. Other polyunsaturated fatty acids namely arachidonic acid, cis, 11, 14-eicosadienoic acid and linolenic acid also supported the sulfoxidation reaction. Nordihydroguaiaretic acid and phenidone, the classical inhibitors of lipoxygenase, significantly blocked the sulfoxidation of thiobenzamide.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP2091396 cis-11,14-Eicosadienoic acid cis-11,14-Eicosadienoic acid 2091-39-6 Price
qrcode