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Role of SDF-1 and Wnt Signaling Pathway in the Myocardial Fibrosis of Hypertensive Rats

Role of SDF-1 and Wnt Signaling Pathway in the Myocardial Fibrosis of Hypertensive Rats

Shuai Shao, Wenwei Cai, Jing Sheng, Lingni Yin

Am J Transl Res. 2015 Aug 15;7(8):1345-56.

PMID: 26396666

Abstract:

Objective:
To investigate the effects of stromal cell-derived factor-1 (SDF-1) and Wnt signaling pathway on the bioactivities of myofibroblasts (MFs) and the expressions of SDF-1 and components of Wnt signaling pathway in the myocardium of spontaneously hypertensive rats (SHR).
Methods:
BMSCs were induced to differentiate into MFs in vitro, and SDF-1 and Wnt signaling pathway were independently or simultaneously blocked. Then, the migration of MFs and the secretion of Col I and α-SMA were determined in MFs. Heart function, progression of myocardial fibrosis and structure of the heart were evaluated. The expression of SDF-1 and components of Wnt signaling pathway in SHR was detected.
Results:
TGF-β could induce the differentiation of BMSCs into B-MFs; Blocking SDF-1/CXCR4 axis and/or Wnt signaling pathway was able to inhibit the MFs migration and Col I secretion; Blocking Wnt signaling pathway inhibited the differentiation of BMSCs into MFs; Serum SDF-1 increased with the increase in blood pressure, and serum β-catenin elevated with the fluctuation of blood pressure; Protein and mRNA expressions of SDF-1 in the myocardium increased, and those of DKK-1 (an inhibitor of Wnt signaling pathway) and GSK-3 reduced in SHR.
Conclusion:
SDF-1 and Wnt signaling pathway are involved in the differentiation of BMSCs into MFs, as well as the migration and collagen secretion of MFs; Hypertension affects the expressions of SDF-1 and components of Wnt signaling pathway. In the myocardium of SHR, SDF-1 expression increases, but the expression of inhibitor of Wnt signaling pathway reduces.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413120	SDF-1 beta from rat			Price

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