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RRAS2 Knockdown Suppresses Osteosarcoma Progression by Inactivating the MEK/ERK Signaling Pathway

RRAS2 Knockdown Suppresses Osteosarcoma Progression by Inactivating the MEK/ERK Signaling Pathway

Kejun Wang, Kan Peng

Anticancer Drugs. 2019 Oct;30(9):933-939.

PMID: 31517733

Abstract:

Aberrant function of RRAS2 drives malignant transformation in a various of cancers. However, little information exists on the function of RRAS2 in tumorigenesis of osteosarcoma. In this study, we investigated the effect of RRAS2 on osteosarcoma progression and its underlying mechanism. The gene expression level and prognostic power of RRAS2 in osteosarcoma were first investigated using the data from the Gene Expression Omnibus database. Then RNA interference was performed to silence the expression of RRAS2 in osteosarcoma cells. Quantitative real-time-PCR and western blot were used to examine the gene and protein expressions of RRAS2 in osteosarcoma cells. In-vitro cancer proliferation and migration were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolum bromide solution and wound-healing assays, respectively. We found that RRAS2 was significantly upregulated in osteosarcoma cells and high expression of RRAS2 was associated with a poor prognosis for patients with osteosarcoma. RNA interference decreased the gene and protein expression of RRAS2, reduced in-vitro the proliferation and migration of osteosarcoma cells, and suppressed the activation of the MEK/ERK signaling pathway. RRAS2 as an adverse prognostic factor promoted cell proliferation and migration by activating the MEK/ERK signaling pathway, and may provide new therapeutic value for osteosarcoma.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP312693188	(S)-(−)-3-Methoxy-2-methyl-3-oxopropylzinc bromide solution		312693-18-8	Price

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