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Ryanodine Receptors Are Expressed in Epidermal Keratinocytes and Associated With Keratinocyte Differentiation and Epidermal Permeability Barrier Homeostasis

Ryanodine Receptors Are Expressed in Epidermal Keratinocytes and Associated With Keratinocyte Differentiation and Epidermal Permeability Barrier Homeostasis

Sumiko Denda, Junichi Kumamoto, Kentaro Takei, Moe Tsutsumi, Hirofumi Aoki, Mitsuhiro Denda

J Invest Dermatol. 2012 Jan;132(1):69-75.

PMID: 21881589

Abstract:

Ryanodine receptors (RyRs) have an important role as calcium channels in the regulation of intracellular calcium levels in the nervous system and muscle. In the present study, we investigated the expression of RyR in human epidermis. Immunohistochemical studies and reverse transcription-PCR indicated the expression of RyR type 1, 2, and 3 proteins in epidermal keratinocytes. The expression level of each RyR subtype was higher in differentiating keratinocytes than in proliferative cells. We also demonstrated the functional expression of RyR by calcium imaging. In cultured human keratinocytes, application of the RyR agonist 4-chloro-m-cresol (CMC) induced elevation of the intracellular calcium concentration, and co-application of the RyR antagonist 1,1'-diheptyl-4,4'-bipyridinium dibromide (DHBP) blocked the elevation. Application of CMC accelerated keratinocyte differentiation in vitro. On the other hand, topical application of CMC after tape-stripping of hairless mouse skin delayed barrier recovery, whereas application of an RyR antagonist, dantrolene or DHBP, accelerated the barrier recovery. These results suggest that RyR expressed in epidermal keratinocytes is associated with both differentiation of keratinocytes and epidermal barrier homeostasis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6159053	1,1′-Diheptyl-4,4′-bipyridinium dibromide		6159-05-3	Price

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