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S-layer protein-AuNP Systems for the Colorimetric Detection of Metal and Metalloid Ions in Water

S-layer protein-AuNP Systems for the Colorimetric Detection of Metal and Metalloid Ions in Water

J Jung, M Lakatos, S Bengs, S Matys, J Raff, A Blüher, G Cuniberti

Colloids Surf B Biointerfaces. 2019 Nov 1;183:110284.

PMID: 31421406

Abstract:

Bacterial surface layer proteins (S-layer) possess unique binding properties for metal ions. By combining the binding capability of S-layer proteins with the optical properties of gold nanoparticles (AuNP), namely plasmonic resonance, a colorimetric detection system for metal and metalloid ions in water was developed. Eight S-layer proteins from different bacteria species were used for the functionalization of AuNP. The thus developed biohybrid systems, AuNP functionalized with S-layer proteins, were tested with different metal salt solutions, e.g. Indium(III)-chloride, Yttrium(III)-chloride or Nickel(II)-chloride, to determine their selective and sensitive binding to ionic analytes. All tested S-layer proteins displayed unique binding affinities for the different metal ions. For each S-layer and metal ion combination markedly different reaction patterns and differences in concentration range and absorption spectra were detected by UV/vis spectroscopy. In this way, the selective detection of tested metal ions was achieved by differentiated analysis of a colorimetric screening assay of these biohybrid systems. A highly selective and sensitive detection of yttrium ions down to a concentration of 1.67 × 10-5 mol/l was achieved with S-layer protein SslA functionalized AuNP. The presented biohybrid systems can thus be used as a sensitive and fast sensor system for metal and metalloid ions in aqueous systems.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP10025828	Indium(III) chloride	10025-82-8	Price
AP10361929	Yttrium(III) chloride	10361-92-9	Price
AP13465117	Indium(II) chloride	13465-11-7	Price

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