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SCD1 and SCD2 Form a Complex That Functions With the Exocyst and RabE1 in Exocytosis and Cytokinesis

SCD1 and SCD2 Form a Complex That Functions With the Exocyst and RabE1 in Exocytosis and Cytokinesis

Jonathan Russell Mayers, Tianwei Hu, Chao Wang, Jessica J Cárdenas, Yuqi Tan, Jianwei Pan, Sebastian Y Bednarek

Plant Cell. 2017 Oct;29(10):2610-2625.

PMID: 28970336

Abstract:

Although exocytosis is critical for the proper trafficking of materials to the plasma membrane, relatively little is known about the mechanistic details of post-Golgi trafficking in plants. Here, we demonstrate that the DENN (Differentially Expressed in Normal and Neoplastic cells) domain protein STOMATAL CYTOKINESIS DEFECTIVE1 (SCD1) and SCD2 form a previously unknown protein complex, the SCD complex, that functionally interacts with subunits of the exocyst complex and the RabE1 family of GTPases in Arabidopsis thaliana Consistent with a role in post-Golgi trafficking, scd1 and scd2 mutants display defects in exocytosis and recycling of PIN2-GFP. Perturbation of exocytosis using the small molecule Endosidin2 results in growth inhibition and PIN2-GFP trafficking defects in scd1 and scd2 mutants. In addition to the exocyst, the SCD complex binds in a nucleotide state-specific manner with Sec4p/Rab8-related RabE1 GTPases and overexpression of wild-type RabE1 rescues scd1 temperature-sensitive mutants. Furthermore, SCD1 colocalizes with the exocyst subunit, SEC15B, and RabE1 at the cell plate and in distinct punctae at or near the plasma membrane. Our findings reveal a mechanism for plant exocytosis, through the identification and characterization of a protein interaction network that includes the SCD complex, RabE1, and the exocyst.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1839524445	Endosidin2		1839524-44-5	Price

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