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Screening, Identification and Quantification of Glucosinolates in Black Radish (Raphanus Sativus L. Niger) Based Dietary Supplements Using Liquid Chromatography Coupled With a Photodiode Array and Liquid Chromatography-Mass Spectrometry

Screening, Identification and Quantification of Glucosinolates in Black Radish (Raphanus Sativus L. Niger) Based Dietary Supplements Using Liquid Chromatography Coupled With a Photodiode Array and Liquid Chromatography-Mass Spectrometry

E Njumbe Ediage, J Diana Di Mavungu, M L Scippo, Y J Schneider, Y Larondelle, A Callebaut, J Robbens, C Van Peteghem, S De Saeger

J Chromatogr A. 2011 Jul 15;1218(28):4395-405.

PMID: 21640355

Abstract:

The glucosinolate profile of black radish (Raphanus sativus L. niger) based dietary supplements has been investigated by HPLC-PDA, LC-ESI-MS/MS and LC-APCI-MS/MS systems. Optimization of the MS/MS parameters and LC conditions was performed using sinigrin reference standard and rapeseed certified reference material (BC190) respectively. An LC-ESI-MS/MS system was used to detect (screen) and identify the naturally occurring intact glucosinolates (GLs). The intact GLs identified were then desulfated and quantified on an HPLC-PDA system as desulfo-glucosinolates (DS-GLs). Prior to quantification, the DS-GLs were identified using an APCI-MS/MS. The HPLC-PDA method performance criteria were evaluated using glucotropaeolin potassium salt. The validated method was applied for the analysis of six dietary supplements. In total, six glucosinolates were identified and quantified in the dietary supplements; glucoraphasatin (0.2-0.48 mg/g), glucosisaustricin (0.37-0.91 mg/g), glucoraphenin (0.84-1.27 mg/g), glucoputrajivin (0.14-0.28 mg/g), glucosisymbrin (0.70-0.99 mg/g) and gluconasturtiin (0.06-0.12 mg/g). Glucoraphenin was the most abundant glucosinolate in all samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP18425768	Gluconasturtiin potassium salt	18425-76-8	Price
AP3952985-B	Sinigrin potassium salt	3952-98-5	Price
AP5115719	Glucotropaeolin potassium salt	5115-71-9	Price

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