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SEC Separation of Polysaccharides Using Macroporous Spherical Silica Gel as a Stationary Phase

SEC Separation of Polysaccharides Using Macroporous Spherical Silica Gel as a Stationary Phase

Tomasz Krawczyk, Mariusz Zalewski, Anna Janeta, Paweł Hodurek

Chromatographia. 2018;81(10):1365-1372.

PMID: 30294004

Abstract:

Abstract:
Meso- and macroporous spherical silica gels of pore sizes in the range of 60-1000 Å and 40-75 µm particle size were investigated as a stationary phase for the separation and purification of polysaccharides and poly(ethylene glycols) (PEGs) of various MWs using an aqueous mobile phase. Sephadex and Bio-Gel were used for comparison as the most common stationary phases for similar purposes. The separation of dextrans of a mean MW = 31 kDa from small molecules (NaCl) was possible with SiO2 with a pore size of 60-300 Å, but the observed efficiencies of a column of the same size were lower comparing with Sephadex or Bio-Gel. In the case of oxidized alginic acid only SiO2 of the 60 Å pore size was suitable, while Sephadex, Bio-Gel and other investigated silicas were not efficient. Sephadex and 300-1000 Å SiO2 offered the possibility of dividing dextrans with MW within the range of 1 MDa-10 kDa into fractions of various MWs, while Bio-Gel and 60 Å SiO2 were not suitable. The investigated silica gels strongly adsorbed PEGs of MW 2-20 kDa. The amount adsorbed decreased with the increase of pore size and they were not useful as a stationary phase for this class of polymers. An advantage of SiO2 of the investigated particle size was a very low back pressure comparing with Sephadex. A considerably lower price of silica offers time- and cost-efficient separation of polysaccharides.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP112926008-A	Silica gel spherical		112926-00-8	Price

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