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Secreted Cyclophilin A Induction During Embryo Implantation in a Model of Human Trophoblast-Endometrial Epithelium Interaction

Secreted Cyclophilin A Induction During Embryo Implantation in a Model of Human Trophoblast-Endometrial Epithelium Interaction

Chih-Liang Tien, Chao-Hsiung Lin, Ting-Hui Lin, Feng-Chen Wen, Chien-Wei Su, Sheng-Seen Fan, Mingli Hsieh

Eur J Obstet Gynecol Reprod Biol. 2012 Sep;164(1):55-9.

PMID: 22677509

Abstract:

Objective:
Embryo implantation in the human is a complex process that is crucial for a successful pregnancy. Implantation involves complex interactions between the embryo and the maternal endometrium. In order to understand the critical events involved in human embryo implantation, we established a human trophoblast-endometrial interaction model to study the putative alterations of gene expression during implantation.
Study design:
Comparative proteomic analysis was applied to the co-culture and separated culture systems of the trophoblast cell line BeWo and human endometrial epithelial cell (EEC) line RL95-2. Comparing the secreted molecules resulted in an interesting finding that secreted cyclophilin A (CypA) was increased in the co-culture system. To further verify our observation, human recombinant CypA was applied to endometrial cells to understand the downstream gene regulation.
Results:
Cyclophilin A (CypA) was identified by MALDI-TOF Mass with higher expression levels in the co-cultured cells when compared to the endometrial cells alone. Western blot analysis further confirmed the increased expression of CypA in co-culture conditions. Immunoblotting analysis showed that both p38 MAPK and extracellular signal-regulated kinase (ERK) were activated in EEC.
Conclusion:
Our results suggest that the secreted CypA plays a specific role in the signaling pathway of embryo implantation. The identification of CypA opens a new avenue for early embryo implantation research.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP95076930	Cyclophilin A human		95076-93-0	Price

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