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Selection of BoCD25 Monoclonal Antibodies by Screening Mouse L Cells Transfected With the Bovine p55-interleukin-2 (IL-2) Receptor Gene

Selection of BoCD25 Monoclonal Antibodies by Screening Mouse L Cells Transfected With the Bovine p55-interleukin-2 (IL-2) Receptor Gene

J Naessens, M Sileghem, N MacHugh, Y H Park, W C Davis, P Toye

Immunology. 1992 Jun;76(2):305-9.

PMID: 1634253

Abstract:

The bovine interleukin-2 receptor-alpha (IL-2R alpha) gene has been isolated and a rabbit antiserum against a fusion protein of the gene has been produced. However, the antiserum does not inhibit IL-2-dependent proliferation. Since a panel of monoclonal antibodies (mAb) to bovine activation antigens was available, we transfected the gene into mouse L fibroblasts, selected stable transfectants with the rabbit antiserum, and screened for antibodies that bound the transfected cells but not the untransfected cells. Three mAb were selected and all three precipitated a molecule of M(r) 55,000 (under reducing conditions) from activated cells, as expected from homology with mouse and human IL-2R alpha (CD25, Tac). One of the three mAb was a strong inhibitor of IL-2-dependent proliferation of bovine lymphocytes. Thus, the availability of transfected cells allowed us to establish quickly and unequivocally the antigenic specificity of a number of antibodies.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42416765	Anti-phospho-Estrogen Receptor-α (pSer305) antibody produced in rabbit			Price
IAR42416787	Anti-phospho-IL-2R α/CD25 (pSer268) antibody produced in rabbit			Price

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