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Selective Detection of Epimeric Pentose Saccharides at Physiological pH Using a Fluorescent Receptor

Matthew I Gibson, João Neres, Elizabeth Fullam

Carbohydr Res. 2014 Jun 4;391:61-5.

PMID: 24785389

Abstract:

Epimerisation between ribofuranose and arabinofuranose sugars is crucial in several biosynthetic pathways, but is typically challenging to monitor. Here, we have screened for fluorescent boronic acids that can be used as molecular probes for the specific detection of ribofuranose over arabinofuranose sugars in solution. We show excellent specificity of the fluorescent response of 3-biphenylboronic acid to ribofuranose at physiological pH. This provides a tool for in situ monitoring of carbohydrate modifying enzymes and provides a viable alternative to traditional radiolabelled assays.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP5122952 3-Biphenylboronic acid 3-Biphenylboronic acid 5122-95-2 Price
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