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Selective Estrogen Receptor Modulator and Prostimulatory Effects of Phytoestrogen β-ecdysone in Tinospora Cordifolia on Osteoblast Cells

Selective Estrogen Receptor Modulator and Prostimulatory Effects of Phytoestrogen β-ecdysone in Tinospora Cordifolia on Osteoblast Cells

Ganesan Abiramasundari, C M Mohan Gowda, Meenakshisundaram Sreepriya

J Ayurveda Integr Med. Jul-Sep 2018;9(3):161-168.

PMID: 30166229

Abstract:

Background:
Indian ethnomedicine acclaims Tinosporacordifolia as a bone strengthening agent and prescribes it for the treatment of bone fractures, gout and other inflammatory diseases of the bone.
Objective:
(a) To understand the potential of T. cordifolia to act as a Selective Estrogen Receptor Modulator (SERM) on in vitro models. (b) To understand the toxic effects (if any) of T. cordifolia in vivo. (c) To understand the effects of β-ecdysone (proposed osteoprotective principle of T. cordifolia) on the growth of human osteoblast-like cells MG-63 and rat primary culture of osteoblasts. (d) To conduct phytochemical analysis on T. cordifolia extract to confirm the presence of β-ecdysone.
Materials and methods:
The role of T. cordifolia as SERM was analyzed by investigating the effect of the extract on the growth of MCF-7 and HeLa cells. The effects of T. cordifolia in vivo was studied by biochemical (Liver function and renal function tests) and histopathological (Hematoxylin/Eosin staining) analysis. Phytochemical analysis of T.cordifolia was carried out by performing FT-IR and LC-ESI-MS analysis.
Results:
(a) T. cordifolia extract exerted non-estrogenic effects on MCF-7 and HeLa cells implicating its role as SERM. (b) High doses of T. cordifolia extract (750 and 1000 mg/kg body wt.) showed impairment of hepatic and renal function, induced pathological alterations in hepatic and renal architecture in albino rats. (c) β-ecdysone an ecdysteroid proposed as the osteoprotective principle of T. cordifolia exhibited significant prostimulatory effects on osteoblast cells and rat primary osteoblasts. (d) Phytochemical analysis confirmed the presence of β-ecdysone in alcoholic extract of T. cordifolia extract substantiating its role as the osteoprotective principle of T. cordifolia.
Conclusion:
(a) T. cordifolia could function as SERM and can have applications in the therapy of osteoporosis. (b) β-ecdysone is the osteoprotective principle of T. cordifolia.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP5289747	β-Ecdysone		5289-74-7	Price

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