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Selective Extraction of 3,4-dihydroxybenzoic Acid in Ilex Chinensis Sims by Meticulous Mini-Solid-Phase Microextraction Using Ternary Deep Eutectic Solvent-Based Molecularly Imprinted Polymers

Selective Extraction of 3,4-dihydroxybenzoic Acid in Ilex Chinensis Sims by Meticulous Mini-Solid-Phase Microextraction Using Ternary Deep Eutectic Solvent-Based Molecularly Imprinted Polymers

Guizhen Li, Kyung Ho Row

Anal Bioanal Chem. 2018 Dec;410(30):7849-7858.

PMID: 30284000

Abstract:

A ternary deep eutectic solvent (TDES) was used as both template and functional monomer in the synthesis of TDES-based molecularly imprinted polymers (TDES-MIPs). A meticulous miniaturized solid-phase microextraction (mini-SPME) method followed by high-performance liquid chromatography (HPLC) were used for the optimal speciation of 3,4-dihydroxybenzoic acid (3,4-DHBA) in the needle of a syringe system with response surface methodology (RSM). Under the optimal conditions for the determination of 3,4-DHBA (amount of adsorbent (2 mg), sample volume (1 mL), cycles for adsorption and desorption (6)), the actual extraction amount was 8.46 μg g-1. The limits of detection (LODs, S/N = 3) for 3,4-DHBA in Ilex chinensis Sims were 0.26-0.31 μg mL-1, and the intra-day and inter-day precision (relative standard deviations, n = 4) after spiking with 5 μg mL-1, 100 μg mL-1, and 200 μg mL-1 were both less than 4.21%. The meticulous method (TDES-MIP-mini-SPME) combined with RSM offers a significant advance over existing methods, because of the meticulous operation and excellent selectivity of 3,4-DHBA from complex samples. Graphical abstract ᅟ.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP99503-A	3,4-Dihydroxybenzoic acid		99-50-3	Price

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