Home
Resources
Publications
Selective Labeling of Embryonic Neurons Cultured on Astrocyte Monolayers With 5(6)-carboxyfluorescein Diacetate (CFDA)

Selective Labeling of Embryonic Neurons Cultured on Astrocyte Monolayers With 5(6)-carboxyfluorescein Diacetate (CFDA)

R E Petroski, H M Geller

J Neurosci Methods. 1994 Apr;52(1):23-32.

PMID: 8090014

Abstract:

A method for selectively labeling cultured neurons using the vital dye, 5(6)-carboxyfluorescein diacetate (CFDA), is described. This non-fluorescent membrane-permeant dye is cleaved by cytosolic esterases into the fluorescent anion, 5(6)-carboxyfluorescein (CF). Both astrocytes and neurons exhibit brilliant fluorochromasia within minutes of CFDA loading. However, following a brief rinse in buffered saline in the absence of CFDA, the astrocytes rapidly lose their cellular fluorescence while the neurons retain the dye for several hours. The fluorochromasia is uniformly distributed throughout the soma and processes which greatly facilitates the morphological identification of viable neurons. In addition, this protocol can be used to conveniently quantify neuronal survival in assays of the activities of neurotrophic or neurotoxic substances.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP124387195	5(6)-Carboxyfluorescein diacetate		124387-19-5	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: