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Semipreparative Capillary Electrochromatography

J R Chen, R N Zare, E C Peters, F Svec, J J Frechét

Anal Chem. 2001 May 1;73(9):1987-92.

PMID: 11354480

Abstract:

Capillaries with inner diameters of 550 microm have successfully been packed with 1.5-microm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 microL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP25639218 Poly(octadecyl methacrylate) solution Poly(octadecyl methacrylate) solution 25639-21-8 Price
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