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Sensitive Detection of endo-1,4-beta-glucanases and endo-1,4-beta-xylanases in Gels

Sensitive Detection of endo-1,4-beta-glucanases and endo-1,4-beta-xylanases in Gels

P Biely, O Markovic, D Mislovicová

Anal Biochem. 1985 Jan;144(1):147-51.

PMID: 3838627

Abstract:

A simple, highly sensitive zymogram technique for detection of endo-1,4-beta-glucanases and endo-1,4-beta-xylanases in polyacrylamide gels after electrophoresis or isoelectric focusing was developed. The detection employs transparent agar replicas containing soluble covalently dyed polysaccharides, hydroxyethylcellulose dyed with Ostazin brilliant red H-3B and beechwood 4-O-methyl-D-glucurono-D-xylan dyed with Remazol brilliant blue R, as the respective substrates. The high sensitivity of the detection is achieved by selective removal of depolymerized dyed substrates from the agar replicas by solvents which neither solubilize nor precipitate the original nondegraded dyed polysaccharides present in the agar gel.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42416024	4-O-Methyl-D-glucurono-D-xylan dyed with Remazol brilliant blue R			Price
LS7413134	Remazol Brilliant Blue R−D-Xylan			Price

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