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Sensitive Determination of a Novel Bisphosphonate, YM529, in Plasma, Urine and Bone by High-Performance Liquid Chromatography With Fluorescence Detection

Sensitive Determination of a Novel Bisphosphonate, YM529, in Plasma, Urine and Bone by High-Performance Liquid Chromatography With Fluorescence Detection

T Usui, R Kawakami, T Watanabe, S Higuchi

J Chromatogr. 1994 Jan 14;652(1):67-72.

PMID: 8014229

Abstract:

A high-performance liquid chromatographic method for the sensitive determination of 1-hydroxy-2-(imidazo[1,2-a]pyridin-3-yl)ethane-1, 1-bisphosphonic acid monohydrate (YM529) in plasma, urine and bone is described. Plasma obtained in high-dose animal studies is treated by method A, a simple method using 1 ml of plasma, which is based on deproteinization of plasma followed by coprecipitation of the drug with calcium phosphate and dissolution of the precipitate in EDTA. Plasma obtained in low-dose clinical studies is treated by method B, a more sensitive method using 4 ml of plasma, which is based on direct precipitation of the drug prior to the deproteinization in method A. Urine and bone samples are prepared by solid-phase extraction using a Sep-Pak C18 cartridge coupled with method A. The drug is separated with a reversed-phase column using a mobile phase at pH 7, and detected with a fluorescence detector following postcolumn alkalization of the mobile phase to enhance fluorescence intensity. The limit of determination is 0.2 ng/ml for method A and 0.05 ng/ml for method B in plasma, 0.05 ng/ml in urine, and 5 ng/g in bone.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP155648605	Minodronic acid monohydrate		155648-60-5	Price

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