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Separation and Identification of the 4-hydroxyantipyrine Sulphoconjugate

Separation and Identification of the 4-hydroxyantipyrine Sulphoconjugate

J Moreau, C Palette, P Cordonnier, E Naline, C Advenier, M Pays

J Chromatogr. 1992 Apr 15;576(1):103-9.

PMID: 1500444

Abstract:

In a previous study we observed, during separation of total antipyrine metabolites by high-performance liquid chromatography and after enzymatic hydrolysis, an unidentified peak corresponding to an ionic compound with pyrazolinone features. In the present study, this compound was identified as the 4-hydroxyantipyrine sulphoconjugate, and its structure was definitively confirmed by gas chromatographic-mass spectrometric analysis and by the use of pure synthetic substance. We also demonstrated the inhibitory effect of sodium metabisulphite, a necessary preservative of urinary samples, on hydrolysis of this conjugate in the presence of sulphatases from Helix pomatia or Aerobacter aerogenes. This inhibitory effect makes it impossible to perform a global assay of antipyrine metabolites after enzymatic or chemical hydrolysis and confirms the value of direct assay of the 4-hydroxyantipyrine sulphoconjugate.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1672635	4-Hydroxyantipyrine		1672-63-5	Price

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