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Separation of Five Flavonoids From Tartary Buckwheat (Fagopyrum Tataricum (L.) Gaertn) Grains via Off-Line Two Dimensional High-Speed Counter-Current Chromatography

Separation of Five Flavonoids From Tartary Buckwheat (Fagopyrum Tataricum (L.) Gaertn) Grains via Off-Line Two Dimensional High-Speed Counter-Current Chromatography

Shujing Jiang, Qi Liu, Yixi Xie, Hualiang Zeng, Li Zhang, Xinyu Jiang, Xiaoqing Chen

Food Chem. 2015 Nov 1;186:153-9.

PMID: 25976805

Abstract:

An off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) strategy was successfully used for preparative separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains with different solvent systems for the first time in this paper. n-Hexane-ethyl acetate-methanol-water 3:5:3:5 (v/v) was selected as the first dimension solvent system to purify quercetin (4) and kaempferol (5). The second dimension solvent system, ethyl acetate-n-butanol-water 7:3:10 (v/v), was used to isolate quercetin 3-O-rutinoside-3'-O-β-glucopyranoside (1), rutin (2) and kaempferol 3-rutinoside (3). The purities of these compounds were all above 96.0% and their structures were identified through UV, MS and (1)H NMR. The results indicated that the off-line 2D HSCCC is an efficient technique to isolate flavonoids compounds from grains.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP17650849	Kaempferol 3-rutinoside		17650-84-9	Price

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