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Separation of Gd-humic Complexes and Gd-based Magnetic Resonance Imaging Contrast Agent in River Water With QAE-Sephadex A-25 for the Fractionation Analysis

Separation of Gd-humic Complexes and Gd-based Magnetic Resonance Imaging Contrast Agent in River Water With QAE-Sephadex A-25 for the Fractionation Analysis

Hiroaki Matsumiya, Hiroto Inoue, Masataka Hiraide

Talanta. 2014 Oct;128:500-4.

PMID: 25059192

Abstract:

Gadolinium complexed with naturally occurring, negatively charged humic substances (humic and fulvic acids) was collected from 500 mL of sample solution onto a column packed with 150 mg of a strongly basic anion-exchanger (QAE-Sephadex A-25). A Gd-based magnetic resonance imaging contrast agent (diethylenetriamine-N,N,N',N″,N″-pentaacetato aquo gadolinium(III), Gd-DTPA(2-)) was simultaneously collected on the same column. The Gd-DTPA complex was desorbed by anion-exchange with 50mM tetramethylammonium sulfate, leaving the Gd-humic complexes on the column. The Gd-humic complexes were subsequently dissociated with 1M nitric acid to desorb the humic fraction of Gd. The two-step desorption with small volumes of the eluting agents allowed the 100-fold preconcentration for the fractionation analysis of Gd at low ng L(-1) levels by inductively coupled plasma-mass spectrometry (ICP-MS). On the other hand, Gd(III) neither complexed with humic substances nor DTPA, i.e., free species, was not sorbed on the column. The free Gd in the effluent was preconcentrated 100-fold by a conventional solid-phase extraction with an iminodiacetic acid-type chelating resin and determined by ICP-MS. The proposed analytical fractionation method was applied to river water samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14190160	Tetramethylammonium sulfate		14190-16-0	Price
AP155788753	Gadolinium(III) sulfate		155788-75-3	Price

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