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Separation of N2-ethyl-2'-deoxyguanosine-5'-monophosphate and Four Native Deoxyribonucleoside Monophosphates Using Capillary Zone Electrophoresis With Polyethylene Glycol as Buffer Additive

Separation of N2-ethyl-2'-deoxyguanosine-5'-monophosphate and Four Native Deoxyribonucleoside Monophosphates Using Capillary Zone Electrophoresis With Polyethylene Glycol as Buffer Additive

Y Esaka, S Inagaki, M Goto, M Sako

Electrophoresis. 2001 Jan;22(1):104-8.

PMID: 11197156

Abstract:

We investigated the separation of five deoxyribonucleoside monophosphates: 2'-deoxyguanosine-5'-monophosphate (dGMP), 2'-deoxyadenosine-5'-monophosphate (dAMP), 2'-deoxycytosine-5'-monophosphate (dCMP), 2'-deoxythymidine-5'-monophosphate (dTMP) and a dGMP adduct possessing N2-ethyl-guanine, which has been noted in relation to mutagenesis of alcohol, using capillary zone electrophoresis (CZE). The concentration of polyethylene glycol (PEG) as a modifier and the pH of the running solutions can efficiently control the observed separation. Interaction of PEG with analytes was quantitatively evaluated. PEG worked effectively as a hydrophobic selector in these separations. The values of pKa of the acidic-NH-groups in the base moieties of dGMP, dTMP, and the dGMP adduct are close to that of boric acid used as buffer of the running solutions. The control of their charge was facilitated, enabling improved separations. A more sufficient and fast separation was achieved by both optimization of pH of the running solutions and PEG concentration compared with that obtained by pH control alone. On-line concentration using a stacking method followed by the PEG-assisted CZE was briefly studied.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP101803036	N2-Ethyl-2′-deoxyguanosine		101803-03-6	Price

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