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Separation of Rare Cell Subpopulations With the Aid of Biotin-Labelled Ligands

Separation of Rare Cell Subpopulations With the Aid of Biotin-Labelled Ligands

M Steinitz, A Livoff, S Tamir, T Brenner

Med Oncol Tumor Pharmacother. 1993;10(1-2):49-52.

PMID: 8258994

Abstract:

A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are first labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10(-4). The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411643	Monoclonal Anti-Biotin antibody produced in mouse			Price

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