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Sequential Electroelution and Mass Spectroscopic Identification of Intact Sodium Dodecyl Sulfate-Proteins Labeled With 5(6)-carboxyfluorescein-N-hydroxysuccinimide Ester

Sequential Electroelution and Mass Spectroscopic Identification of Intact Sodium Dodecyl Sulfate-Proteins Labeled With 5(6)-carboxyfluorescein-N-hydroxysuccinimide Ester

S Yefimov, A L Yergey, A Chrambach

Electrophoresis. 2001 Aug;22(14):2881-7.

PMID: 11565783

Abstract:

A gel electrophoresis apparatus capable of scanning the migration path fluorometrically and of computer-directed electroelution of bands was applied to the mass spectrometric identification of sequentially electroeluted 5(6)-carboxyfluorescein-N-hydrosuccinimide ester (FLUOS)-labeled sodium dodedyl sulfate (SDS)-proteins. The masses of four electroeluted SDS-proteins under study determined by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) spectrometry are changed by 1% due to their reaction with FLUOS in a 1:5 molar ratio of protein:label, allowing for the identification of the labeled intact proteins on the basis of mass. More importantly, the partial (10 or 50%) derivatization of proteins with FLUOS does not preclude their tryptic hydrolysis, and identification of the protein on the basis of the mass spectrometric analysis of its tryptic peptides. Potentially, the procedure allows for the automated mass spectrometric identification of SDS-proteins globally labeled with FLUOS and electrophoretically separated, without need for any gel sectioning.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP117548228	5(6)-Carboxyfluorescein N-hydroxysuccinimide ester		117548-22-8	Price

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