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Short Communication: Bovine Alpha-Casein Is a Ferritin-Binding Protein and Inhibitory Factor of Milk Ferritin Immunoassay

Short Communication: Bovine Alpha-Casein Is a Ferritin-Binding Protein and Inhibitory Factor of Milk Ferritin Immunoassay

G Sugawara, R Inoue, K Watanabe, H Ohtsuka, K Orino

J Dairy Sci. 2009 Aug;92(8):3810-4.

PMID: 19620663

Abstract:

Commercial bovine milk alpha-casein, but not beta- and kappa-caseins, bound to bovine spleen ferritin, as determined by an immunoassay for ferritin. In contrast, alpha-casein did not bind to apoferritin. The binding of alpha-casein to bovine spleen ferritin was strongly inhibited by increasing ionic strength by the addition of 0.5 M (NH(4))(2)SO(4). The addition of alpha-casein to a known amount of bovine spleen ferritin resulted in significantly lower recovery (78-80%) of added ferritin, although beta- and kappa-caseins showed little inhibitory effect in the ferritin immunoassay. These results indicate that bovine alpha-casein is a specific ferritin-binding protein that may inhibit milk ferritin immunoassay.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9000719-C	α-Casein from bovine milk		9000-71-9	Price

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