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SICT: automated detection and supervised inspection of fast Ca 2+ transients

SICT: automated detection and supervised inspection of fast Ca 2+ transients

Roberta Mancini, Tobias van der Bijl, Quentin Bourgeois-Jaarsma, Rizky Lasabuda, Alexander J Groffen

Sci Rep. 2018 Oct 19;8(1):15523.

PMID: 30341397

Abstract:

Recent advances in live Ca2+ imaging with increasing spatial and temporal resolution offer unprecedented opportunities, but also generate an unmet need for data processing. Here we developed SICT, a MATLAB program that automatically identifies rapid Ca2+ rises in time-lapse movies with low signal-to-noise ratios, using fluorescent indicators. A graphical user interface allows visual inspection of automatically detected events, reducing manual labour to less than 10% while maintaining quality control. The detection performance was tested using synthetic data with various signal-to-noise ratios. The event inspection phase was evaluated by four human observers. Reliability of the method was demonstrated in a direct comparison between manual and SICT-aided analysis. As a test case in cultured neurons, SICT detected an increase in frequency and duration of spontaneous Ca2+ transients in the presence of caffeine. This new method speeds up the analysis of elementary Ca2+ transients.

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IAR4243291	Fluorescent Adenosine receptor Antagonist (A-633-AN) synthetic			Price

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