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Silicalite-1 Layers as a Biocompatible Nano- And Micro-Structured Coating: An In Vitro Study on MG-63 Cells

Silicalite-1 Layers as a Biocompatible Nano- And Micro-Structured Coating: An In Vitro Study on MG-63 Cells

Martina Doubkova, Ivana Nemcakova, Ivan Jirka, Vitezslav Brezina, Lucie Bacakova

Materials (Basel). 2019 Oct 31;12(21):3583.

PMID: 31683581

Abstract:

Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH2O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP682019	Tetrapropyl orthosilicate		682-01-9	Price

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