Home
Resources
Publications
Simple and Rapid Quantitative Assay of 13C-labelled Urea in Human Serum Using Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

Simple and Rapid Quantitative Assay of 13C-labelled Urea in Human Serum Using Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

T Tanigawa, Y Mizo-oku, K Moriguchi, T Suzuki, T Osumi, M Odomi

J Chromatogr B Biomed Appl. 1996 Aug 30;683(2):135-42.

PMID: 8891910

Abstract:

A simple and rapid quantitative method for 13C-labelled urea ([13C]urea) in human serum was developed by using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS). This method is used to establish and normalize the [13C]urea breath test, which is considered as an effective diagnostic method for Helicobacter pylori infection. HPLC-APCI-MS, involving a simple pretreatment process such as diluting serum with water, was shown to be able to discriminate the extrinsic [13C]urea from intrinsic urea present at high concentration in serum. In addition, a 13C nuclear magnetic resonance spectroscopic quantitative method for [13C]urea in human urine is also described. The precision and accuracy of measured concentrations in these two methods were found to be within the acceptable limit. An application of these methods to investigate the pharmacokinetic profile of orally administered [13C]urea in human serum and urine is also presented.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP58069822-B	Urea-13C		58069-82-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: