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Simple and Rapid Separation of Soyasaponin Bb From a Soy Extract

Simple and Rapid Separation of Soyasaponin Bb From a Soy Extract

Hajime Katano, Nobuhiro Okamoto, Masahiro Takakuwa, Shu Taira, Taiho Kambe, Masakazu Takahashi

Anal Sci. 2015;31(2):85-9.

PMID: 25746805

Abstract:

A simple method to separate soyasaponin Bb from a soy extract is presented. This method is based on the difference in the solubility of soyasaponin Bb and Ba and other components into 3:7 and 1:1 (v/v) acetone-water mixed solvents. The crude soyasaponin consisting of soyasaponins Aa, Ab, Ba, and Bb at the 10 wt% level and other components was examined as the soy extract. A 10 mg quantity of the crude soyasaponin was mixed with 1 mL of the 3:7 acetone-water containing 0.1 mol/L HCl, and the supernatant was removed to obtain a precipitate, which was found to contain mainly soyasaponins Bb and Ba. The precipitate was mixed with 0.4 mL of the 1:1 acetone-water containing 0.1 mol/L HCl; the supernatant was transferred, and was mixed with 0.6 mL of water to obtain a precipitate, which was found to contain mainly soyasaponin Bb. The yield was ca. 30%, which may be much higher than that by the conventional preparative chromatographic approach. The separation method is rapid and easy to carry out, and is useful for the preparation of a soyasaponin Bb sample.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP51330279	Soyasaponin Bb		51330-27-9	Price

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