Home
Resources
Publications
Simple Defined Autoinduction Medium for High-Level Recombinant Protein Production Using T7-based Escherichia Coli Expression Systems

Simple Defined Autoinduction Medium for High-Level Recombinant Protein Production Using T7-based Escherichia Coli Expression Systems

Zhaopeng Li, Wolfgang Kessler, Joop van den Heuvel, Ursula Rinas

Appl Microbiol Biotechnol. 2011 Aug;91(4):1203-13.

PMID: 21698378

Abstract:

Protein production under the control of lac operon regulatory elements using autoinduction is based on diauxic growth of Escherichia coli on lactose after consumption of more preferred carbon substrates. A novel simple and cost-effective defined autoinduction medium using a mixture of glucose, glycerol, and lactose as carbon substrate and NH(4)(+) as sole nitrogen source without any supplementation of amino acids and vitamins was developed for T7-based E. coli expression systems. This medium was successfully employed in 96-well microtiter plates, test tubes, shake flasks, and 15-L bioreactor cultivations for production of different types of proteins achieving an average yield of 500 mg L(-1) product. Cell-specific protein concentrations and solubility were similar as during conventional isopropyl β-D-1-thiogalactopyranoside induction using Luria-Bertani broth. However, the final yield of target proteins was about four times higher, as a higher final biomass was achieved using this novel defined autoinduction broth.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP367931-B	Isopropyl β-D-1-thiogalactopyranoside		367-93-1	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: