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Simple Gas Chromatographic Method for the Determination of Medicagenic Acid in Alfalfa (Medicago Sativa)

D Rao, G Bories

J Chromatogr. 1987 Nov 20;410(1):169-75.

PMID: 3429547

Abstract:

A gas chromatographic method for the determination of medicagenic acid in alfalfa leaves, stems, entire plant (tops) and roots and also in leaf protein concentrates is described. The method is based on extraction of lucerne saponins followed by hydrolysis of the triterpene glycosides. After derivatization of the liberated sapogenins to silylated products, the trimethylsilylated medicagenic acid was determined by gas chromatography. The sensitivity of the method permits the detection of 50 ng of medicagenic acid.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP599075 Medicagenic acid Medicagenic acid 599-07-5 Price
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