Home
Resources
Publications
Simple Surface Modification of Poly(dimethylsiloxane) for DNA Hybridization

Simple Surface Modification of Poly(dimethylsiloxane) for DNA Hybridization

Jinwen Zhou, Nicolas H Voelcker, Amanda V Ellis

Biomicrofluidics. 2010 Dec 6;4(4):46504.

PMID: 21264061

Abstract:

Here, we present a simple chemical modification of poly(dimethylsiloxane) (PDMS) by curing a mixture of 2 wt% undecylenic acid (UDA) in PDMS prepolymer on a gold-coated glass slide. This gold slide had been previously pretreated with a self-assembled hydrophilic monolayer of 3-mercaptopropionic acid (MPA). During curing of the UDA∕PDMS prepolymer, the hydrophilic UDA carboxyl moieties diffuses toward the hydrophilic MPA carboxyl moieties on the gold surface. This diffusion of the UDA within the PDMS prepolymer to the surface is a direct result of surface energy minimization. Once completely cured, the PDMS is peeled off the gold substrate, thereby exposing the interfacial carboxyl groups. These groups are then available for subsequent attachment of 5(')-amino terminated DNA oligonucleotides via amide linkages. Our results show that the covalently tethered oligonucleotides can successfully capture fluorescein-labeled complementary oligonucleotides via hybridization, which are visualized using fluorescence microscopy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS71607	Poly(dimethylsiloxane), monoacrylamidopropyl terminated			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: