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Simplified Derivatization for Determining Sphingolipid Fatty Acyl Composition by Gas Chromatography-Mass Spectrometry

Simplified Derivatization for Determining Sphingolipid Fatty Acyl Composition by Gas Chromatography-Mass Spectrometry

S B Johnson, R E Brown

J Chromatogr. 1992 Jul 17;605(2):281-6.

PMID: 1500464

Abstract:

A simple procedure for simultaneously derivatizing non-hydroxy and hydroxy fatty acids prior to GC analysis [I. Ciucanu and F. Kerek, J. Chromatogr., 284 (1984) 179] has been evaluated for its usefulness in determining sphingolipid acyl composition. The method uses methyl iodide in polar aprotic solvents to generate methyl esters of carboxyl groups and methyl ethers of hydroxyl groups. Methylation efficiency is examined as a function of hydroxyl group presence and location in free fatty acids as well as a function of 2-hydroxy fatty acid chain length. Conditions are also reported for efficient saponification and derivatization of sphingolipid fatty acyl chains as is illustrated using bovine brain galactosylceramide.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4244178	Non-hydroxy fatty acid ceramide from bovine brain			Price

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