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Simultaneous Determination of 2-deoxy-D-glucose and D-glucose in Rat Serum by High-Performance Liquid Chromatography With Post-Column Fluorescence Derivatization

Y Umegae, H Nohta, Y Ohkura

Chem Pharm Bull (Tokyo). 1990 Apr;38(4):963-5.

PMID: 2379291

Abstract:

A simple high-performance liquid chromatographic method for the determination of 2-deoxy-D-glucose and D-glucose in rat serum is described; this method is based on a post-column fluorescence derivatization. The sugars are automatically converted into fluorescent derivatives by reaction with meso-1,2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium after their separation on a strong anion exchanger column (TSK gel Sugar AXG). The detection limits (S/N = 3) for 2-deoxy-D-glucose and D-glucose in rat serum are 0.52 and 0.56 nmol/ml, respectively.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP58520459 meso-1,2-Bis(4-methoxyphenyl)ethylenediamine meso-1,2-Bis(4-methoxyphenyl)ethylenediamine 58520-45-9 Price
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