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Simultaneous Determination of Aliskiren Hemifumarate, Amlodipine Besylate, and Hydrochlorothiazide in Their Triple Mixture Dosage Form by Capillary Zone Electrophoresis

Simultaneous Determination of Aliskiren Hemifumarate, Amlodipine Besylate, and Hydrochlorothiazide in Their Triple Mixture Dosage Form by Capillary Zone Electrophoresis

Mohamed M Salim, Walid M Ebeid, Nahed El-Enany, Fathalla Belal, Mohamed Walash, Gabor Patonay

J Sep Sci. 2014 May;37(9-10):1206-13.

PMID: 24574149

Abstract:

A novel, specific, reliable, and accurate capillary zone electrophoretic method was developed and validated for the simultaneous determination of aliskiren hemifumarate, amlodipine besylate, and hydrochlorothiazide in their triple mixture dosage form. Separation was carried out in a fused-silica capillary (57.0 cm total length and 50.0 cm effective length, 75.6 μm internal diameter) by applying a potential of 17 kV and a running buffer consisting of 40 mM phosphate buffer at pH 6.0 with UV detection at 245 nm. The method was suitably validated with respect to specificity, linearity, LOD, and LOQ, accuracy, precision, and robustness. The method showed good linearity in the ranges 1-10, 2.5-25, and 30-300 μg/mL with LODs of 0.11, 0.33, and 5.83 μg/mL for amlodipine besylate, hydrochlorothiazide, and aliskiren hemifumarate, respectively. The proposed method was successfully applied for the analysis of the studied drugs in their coformulated tablets. The results of the proposed method were statistically compared with those obtained by the RP-HPLC reference method revealing no significant differences in the performance of the methods regarding accuracy and precision.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP173334582	Aliskiren hemifumarate		173334-58-2	Price

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