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[Simultaneous Determination of Aspartame and Alitame in Jellies and Preserved Fruits by HPLC]

[Simultaneous Determination of Aspartame and Alitame in Jellies and Preserved Fruits by HPLC]

Dingguo Jiang, Congrong Fang, Dajin Yang

Wei Sheng Yan Jiu. 2012 May;41(3):467-70, 475.

PMID: 23050451

Abstract:

Objective:
To establish a determination method for aspartame and alitame in jellies and preserved fruits.
Methods:
Aspartame and alitame in jellies were extracted with 80% methanol at 70 degrees C. Preserved fruits were homogenized with water and then were extracted with 50% methanol. A chromatographic column Zorbax SB-C18 was used; the mobile phase was methanol/water (40 + 60, volume ratio), and a diode array detector was used for the detection at wavelength 200 nm.
Results:
The limits of quantification of aspartame and alitame were both 8 mg/kg for jellies and both 20 mg/kg for preserved fruits; the calibration curves were linear in the range of tested concentration. The correlation coefficients were better than 0.9996. The average recovery rates were in the range of 98.1% -101.2%, the relative standard deviations were 2.21% - 4.10%.
Conclusion:
The method is simple, practical, accurate, reliable and successful in the determination of aspartame and alitame in jellies and preserved fruits from various brands on markets.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP99016429	Alitame		99016-42-9	Price

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