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Simultaneous Determination of Aurantio-Obtusin, Chrysoobtusin, Obtusin and 1-desmethylobtusin in Rat Plasma by UHPLC-MS/MS

Wei-dong Zhang, Peng-yuan Wang, Ying Wang, Qing Wang, Yi Gu, Jun Cao, Shao-qing Wang, Xiao-juan Wang

Biomed Chromatogr. 2014 Mar;28(3):369-74.

PMID: 24027008

Abstract:

A sensitive and reliable ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the simultaneous determination of four active components of Semen Cassiae extract (aurantio-obtusin, chrysoobtusin, obtusin and 1-desmethylobtusin) in rat plasma after oral administration. Chromatographic separation was achieved on an Agilent Poroshell 120 C18 column with gradient elution using a mobile phase that consisted of acetonitrile-ammonium acetate in water (30 mm) at a flow rate of 0.4 mL/min. Detection was performed by a triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The calibration curve was linear over a range of 3.24-1296 ng/mL for aurantio-obtusin, 0.77-618 ng/mL for chrysoobtusin, 34.55-1818 ng/mL for obtusin and 1.86-1485 ng/mL for 1-desmethylobtusin. Inter- and intra-day assay variation was <15%. All analytes were shown to be stable during all sample storage and analysis procedures.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP67979253 Aurantio-obtusin Aurantio-obtusin 67979-25-3 Price
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