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[Simultaneous Determination of Chlorogenic Acid, Cryptochlorogenic Acid, Caffeic Acid, Naringin, Hesperidin and Linarin in Xiao'erjinning Oral Liquid by an HPLC Method]

[Simultaneous Determination of Chlorogenic Acid, Cryptochlorogenic Acid, Caffeic Acid, Naringin, Hesperidin and Linarin in Xiao'erjinning Oral Liquid by an HPLC Method]

Jing Wang, Xinzhuang Zhang, Xiaoyan Wu, Xiaoli Zhao, Liuqing Di, Qing Guo, Shouchuan Wang

Zhongguo Zhong Yao Za Zhi. 2010 Jul;35(13):1702-5.

PMID: 20862961

Abstract:

Objective:
To develop a HPLC method for the simultaneous determination of chlorogenic acid, cryptochlorogenic acid, caffeic acid, naringin, hesperidin and linarin in xiao' erjinning oral liquid.
Method:
The chromatographic separation was achieved on a Lichrospher C18 (4.6 mm x 250 mm, 5 microm) column with a mobile phase which was composed of acetonitrile(A) and 0.4% phosphoric acid(B) for gradient elution (10:90-18:82-27:73). The flow rate was (0.8-1.1-0.8) mL x min(-1), the column temperature was 30 degrees C and the detection wavelength was set at 300 nm.
Result:
The results showed that 6 effective components were separated well and showed good linearity. The average recoveries were between 95%-105%.
Conclusion:
The method is proved to be rapid, accurate, credible and repeatable. It can be used for the quality control of Xiao'erjinning oral liquid.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP905997	Cryptochlorogenic acid		905-99-7	Price

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