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Simultaneous Determination of Cimicifugoside H-2, Cimicifugoside H-1, 23-epi-26-deoxyactein, Cimigenol Xyloside and 25-O-acetylcimigenoside in Beagle Dog Plasma by LC-MS/MS

Simultaneous Determination of Cimicifugoside H-2, Cimicifugoside H-1, 23-epi-26-deoxyactein, Cimigenol Xyloside and 25-O-acetylcimigenoside in Beagle Dog Plasma by LC-MS/MS

Yinglin Wang, Chunjie Sha, Wanhui Liu, Yunyun Gai, Huayun Zhang, Hualing Qu, Wensheng Wang

J Pharm Biomed Anal. 2012 Mar 25;62:87-95.

PMID: 22285707

Abstract:

A selective and sensitive LC-MS/MS method was developed and validated for the simultaneous determination of five constituents (cimicifugoside H-2, cimicifugoside H-1, 23-epi-26-deoxyactein, cimigenol xyloside and 25-O-acetylcimigenoside) of Cimicifuga foetida L. in beagle dog plasma. The quantitation was performed on a LC-MS/MS with negative electrospray ionization in selected reaction monitoring (SRM) mode. A gradient mobile phase composed of methanol and water was used at a flow rate of 0.4 ml/min. All the analytes and internal standard (20 (S)-ginsenoside Rg3) were isolated from plasma samples by a liquid-liquid extraction method. The average extraction recoveries were 73-74% for cimicifugoside H-2, 89-94% for cimicifugoside H-1, 73-80% for 23-epi-26-deoxyactein, 89-91% for cimigenol xyloside, 87-96% for 25-O-acetylcimigenoside, respectively. The method showed good linearity and no endogenous material interfered with all the five compounds and I.S. peaks. The lower limit of quantification (LLOQ) of all analytes was 0.5 ng/ml. The intra- and inter-day precision of analysis was less than 15% for each analyte at concentrations of 2.0, 50, 500 ng/ml, and the accuracy ranged from 85.8% to 107%. This method was successfully applied to reveal the pharmacokinetic properties of cimicifugoside H-2, cimicifugoside H-1, 23-epi-26-deoxyactein, cimigenol xyloside and 25-O-acetylcimigenoside after oral administration.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP27994112	Cimigenol 3-β-D-xyloside		27994-11-2	Price
AP501938018	23-epi-26-Deoxyactein		501938-01-8	Price

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