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Simultaneous Determination of Mangiferin and Neomangiferin in Rat Plasma by UPLC-MS/MS and Its Application for Pharmacokinetic Study

Simultaneous Determination of Mangiferin and Neomangiferin in Rat Plasma by UPLC-MS/MS and Its Application for Pharmacokinetic Study

Xiangjun Qiu, Jian-Long Zhao, Cong Hao, Canli Yuan, Nuan Tian, Zhi-Sheng Xu, Ruan-Min Zou

J Pharm Biomed Anal. 2016 May 30;124:138-142.

PMID: 26945635

Abstract:

In this study, a sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine mangiferin and neomangiferin in rat plasma simultaneously. Chromatographic separation was carried out on an Acquity UPLC BEH C18 column and mass spectrometric analysis was performed using a Xevo TQD triple quadruple mass spectrometer coupled with an electrospray ionization (ESI) source. The MRM transitions of m/z 423.2 → 303.1 and m/z 585.0 → 273.1 were used to quantify for mangiferin and neomangiferin, respectively. The linearity of this method was found to be within the concentration range of 5-2000 ng/mL for mangiferin, and 2-1000 ng/mL for neomangiferin in rat plasma, respectively. Only 3.0 min was needed for an analytical run. This assay was used to support a preclinical study to investigate the pharmacokinetics of mangiferin and neomangiferin in rats.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP64809672	Neomangiferin		64809-67-2	Price

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