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Simultaneous Determination of Metacavir and Its Metabolites in Rat Plasma Using High-Performance Liquid Chromatography With Tandem Mass Spectrometric Detection (LC-MS/MS)

Yue Zhang, Xin Huang, Zhenzhou Jiang, Xiao Huang, Yue Hu, Dan Zhu, Shuang Zhang, Jiaying Wang, Luyong Zhang

Biomed Chromatogr. 2012 Mar;26(3):363-70.

PMID: 21773980

Abstract:

A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of metacavir and its two metabolites in rat plasma was developed and validated. Tinidazole was used as an internal standard and plasma samples were pretreated with one-step liquid-liquid extraction. In addition, these analytes were separated using an isocratic mobile phase on a reverse-phase C18 column and analyzed by MS in the selected reaction monitoring mode. The monitored precursor to product-ion transitions for metacavir, 2',3'-dideoxyguanosine, O-methylguanine and the internal standard were m/z 266.0 → 166.0, m/z 252.0 → 152.0, m/z 166.0 → 149.0 and m/z 248.0 → 202.0, respectively. The standard curves were found to be linear in the range of 1-1000 ng/mL for metacavir, 5-5000 ng/mL for 2',3'-dideoxyguanosine and 1-1000 ng/mL for O-methylguanine in rat plasma. The precision and accuracy for both within- and between-batch determination of all analytes ranged from 2.83 to 9.19% and from 95.86 to 111.27%, respectively. No significant matrix effect was observed. This developed method was successfully applied to an in vivo pharmacokinetic study after a single intravenous dose of 20 mg/kg metacavir in rats.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
CS31042270 Methoxymethylguanine Methoxymethylguanine Price
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