Home
Resources
Publications
Simultaneous Determination of Moexipril Hydrochloride and Hydrochlorothiazide in Tablets by Derivative Spectrophotometric and High-Performance Liquid Chromatographic Methods

Simultaneous Determination of Moexipril Hydrochloride and Hydrochlorothiazide in Tablets by Derivative Spectrophotometric and High-Performance Liquid Chromatographic Methods

Sidika Ertürk, Sevil Müge Cetin, Sedef Atmaca

J Pharm Biomed Anal. 2003 Oct 15;33(3):505-11.

PMID: 14550868

Abstract:

Two new simple and selective assay methods have been presented for the binary mixtures of moexipril hydrochloride (MOEX) and hydrochlorothiazide (HCTZ) in pharmaceutical formulations. The first method depends on second-derivative ultraviolet spectrophotometry with zero-crossing measurements at 215 and 234 nm for MOEX and HCTZ, respectively. The assay was linear over the concentration ranges 1.0-11.0 microg ml(-1) for MOEX and 0.5-9.0 microg ml(-1) for HCTZ. The determination limits for MOEX and HCTZ were found to be 1.0 and 0.5 microg ml(-1), respectively; while the detection limits were 0.2 microg ml(-1) for MOEX and 0.1 microg ml(-1) for HCTZ. The second method was based on isocratic reversed-phase liquid chromatography by using a mobile phase acetonitrile-20 mM phosphate buffer (pH 4.0) (50:50, v/v). Lisinopril was used as an internal standard (IS) and the substances were detected at 212 nm. The linearity range for both drugs was 0.5-12.0 microg ml(-1). The determination and detection limits were found to be 0.100 and 0.010 microg ml(-1) for MOEX and 0.025 and 0.005 microg ml(-1) for HCTZ, respectively. The proposed methods were successfully applied to the determination of these drugs in synthetic mixtures and commercially available tablets with a high percentage recovery, good accuracy and precision.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP82586525	Moexipril hydrochloride		82586-52-5	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: