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Simultaneous Determination of Naproxen and Related Compounds by HPLC Using Porous Graphitic Carbon Column

Simultaneous Determination of Naproxen and Related Compounds by HPLC Using Porous Graphitic Carbon Column

Lotfi Monser, Frida Darghouth

J Pharm Biomed Anal. 2003 Aug 8;32(4-5):1087-92.

PMID: 12899998

Abstract:

A simple, selective and sensitive high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of naproxen and its main degradation products such as 1-(6-methoxy-2-naphthyl) ethanol (MNE), 2-methoxy-6-ethyl naphthalene (MEN) and 2-acetyl-6-methoxy naphthalene (AMN). The separation of these compounds was achieved on porous graphitic carbon (PGC) column using tetrahydrofuran-methanol as the mobile phase, and the effluent from the column was monitored at 272 nm. At a flow rate of 1 ml min(-1), the retention time of the last eluting compound was less than 10 min. Correlation coefficient for calibration curves in the ranges 2-25 microg ml(-1) for all compounds studied were greater than 0.999. The sensitivity of detection is 0.05 microg l(-1) for naproxen, MNE and MEN and 0.20 microg ml(-1) for AMN. The reproducibility of the peak area of these compounds using isocratic elution were quite high, and the standard deviations (S.D.) were below 2% (n=5). The reproducibility of retention times of these compounds was within 1% (n=5). The proposed liquid chromatographic method was successfully applied to the analysis of commercially available naproxen sodium (NS) dosage forms with recoveries of 98.8-102%. A comparative study shows that the selectivity of these compounds on PGC column was different to that obtained with octadecyl silica (ODS) columns.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP3900456	Naproxen Related Compound L		3900-45-6	Price

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