Home
Resources
Publications
Simultaneous Determination of Nortriptyline Hydrochloride and Fluphenazine Hydrochloride in Microgram Quantities From Low Dosage Forms by Liquid chromatography-UV Detection

Simultaneous Determination of Nortriptyline Hydrochloride and Fluphenazine Hydrochloride in Microgram Quantities From Low Dosage Forms by Liquid chromatography-UV Detection

Safwan Ashour, Nuha Kattan

J Pharm Anal. 2012 Dec;2(6):437-442.

PMID: 29403779

Abstract:

A novel method for the simultaneous high-performance liquid chromatographic determination of nortriptyline hydrochloride and fluphenazine hydrochloride was developed and validated. Fluvastatin sodium was used as internal standard. The determination was performed on a Hypersil Gold C8 column (250 mm × 4.6 mm i.d., 5 μm particle size) at 25 °C; the mobile phase, consisting of a mixture of formic acid (0.1 M, pH 2.16)-methanol (33:67, v/v), was delivered at a flow rate of 1.1 mL/min and detector wavelength at 251 nm. The retention time of nortriptyline, fluphenazine and fluvastatin was found to be 5.11, 8.05 and 11.38 min, respectively. Linearity ranges were 5.0-1350.0 and 10.0-1350.0 μg/mL with limit of detection values of 0.72 and 0.31 μg/mL, for nortriptyline and fluphenazine, respectively. Results of assay and recovery studies were statistically evaluated for its accuracy and precision. Correlation coefficients (r2) of the regression equations were greater than 0.999 in all cases. According to the validation results, the proposed method was found to be specific, accurate, precise and could be applied to the simultaneous quantitative analysis of nortriptyline and fluphenazine.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP146565-B	Fluphenazine Hydrochloride		146-56-5	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: