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Simultaneous Determination of P-Aminohippuric Acid, Acetyl-P-Aminohippuric Acid and Iothalamate in Human Plasma and Urine by High-Performance Liquid Chromatography

Simultaneous Determination of P-Aminohippuric Acid, Acetyl-P-Aminohippuric Acid and Iothalamate in Human Plasma and Urine by High-Performance Liquid Chromatography

T C Dowling, R F Frye, M A Zemaitis

J Chromatogr B Biomed Sci Appl. 1998 Sep 25;716(1-2):305-13.

PMID: 9824245

Abstract:

A sensitive and specific high-performance liquid chromatographic assay was developed for the simultaneous determination of p-aminohippuric acid (PAH), acetyl-p-aminohippuric acid (aPAH), and iothalamate in human plasma and urine. Plasma samples were prepared by protein precipitation with acetonitrile followed by evaporation, reconstitution in mobile phase, and injection onto a C18 reversed-phase column. Urine samples were diluted with 3 volumes of mobile phase prior to injection. Column effluent was monitored by UV detection at 254 nm. The lower limits of quantification in plasma were 0.5 mg/l for PAH and aPAH, and 1.0 mg/l for iothalamate. The within-day and between-day coefficients of variation in plasma and urine were < or =7.8% for all analytes. This method is well suited for renal function studies using iothalamate and PAH, whether administered as a bolus dose or by continuous infusion, to measure glomerular filtration rate and effective renal plasma flow, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP61789	Aminohippuric acid		61-78-9	Price

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