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[Simultaneous Determination of Peimisine and Sipeimine in Fritillaria Walujewii Regel and Fritillaria Pallidiflora Schrenk by UPLC-ELSD]

[Simultaneous Determination of Peimisine and Sipeimine in Fritillaria Walujewii Regel and Fritillaria Pallidiflora Schrenk by UPLC-ELSD]

Bao-zhong Duan, Lin-fang Huang, Shi-lin Chen

Yao Xue Xue Bao. 2010 Dec;45(12):1541-4.

PMID: 21351494

Abstract:

The paper reports the establishment of a method for simultaneous determination of peimisine and sipeimine contents in Fritillaria walujewii Regel and Fritillaria pallidiflora Schrenk. The analyses were performed on an ultra-performance liquid chromatography with evaporative light scattering detection (UPLC-ELSD), equipped with a binary solvent manager, a sampler manager and a column compartment, and connected to Waters Empower 2 software. An Acquity UPLC BEH C18 column (100 mm x 2.1 mm, 1.7 microm) was used for all analysis. The investigated compounds were separated with gradient mobile phase consisting of acetonitrile-0.02% triethylamine-water. The temperature of sample manager was set at 25 degrees C. Drift tube temperature was 40 degrees C, and spray parameter was 40% with injection volume of 1 microL. The investigated compounds including peimisine and sipeimine had good linearity (r > or = 0.9991) over the tested ranges. The average recovery was 94.5% and 98.1% with RSD < or = 2.36%. The UPLC-ELSD method is simple, sensitive and accurate with good repeatability, which is available for quality control of F. walujewii Regel and F. pallidiflora Schrenk.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP61825987	Sipeimine		61825-98-7	Price

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