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Simultaneous Determination of Phosphatidylglycerol and the lecithin/sphingomyelin Ratio in Amniotic Fluid

G R Gotelli, R E Stanfill, P M Kabra, F A Farina, L J Marton

Clin Chem. 1978 Jul;24(7):1144-6.

PMID: 77745

Abstract:

We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
DR5605 Molybdenum Blue spray reagent Molybdenum Blue spray reagent Price
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