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Simultaneous Determination of Six HIV Protease Inhibitors (Amprenavir, Indinavir, Lopinavir, Nelfinavir, Ritonavir and Saquinavir), the Active Metabolite of Nelfinavir (M8) and Non-Nucleoside Reverse Transcriptase Inhibitor (Efavirenz) in Human Plasma by High-Performance Liquid Chromatography

Simultaneous Determination of Six HIV Protease Inhibitors (Amprenavir, Indinavir, Lopinavir, Nelfinavir, Ritonavir and Saquinavir), the Active Metabolite of Nelfinavir (M8) and Non-Nucleoside Reverse Transcriptase Inhibitor (Efavirenz) in Human Plasma by High-Performance Liquid Chromatography

Yoshihiro Hirabayashi, Kiyoto Tsuchiya, Satoshi Kimura, Shinichi Oka

Biomed Chromatogr. 2006 Jan;20(1):28-36.

PMID: 15971289

Abstract:

We report the development of a simple, economical and reliable chromatographic method for the simultaneous determination of six HIV protease inhibitors (PIs; amprenavir, indinavir, lopinavir, nelfinavir, ritonavir and saquinavir), the active metabolite of nelfinavir (M8) and the non-nucleoside reverse transcriptase inhibitor (NNRTI; efavirenz) in human plasma. After extraction from plasma with an ethyl acetate-acetonitrile mixture, the analytes were separated on a phenyl column with a gradient of acetonitrile and phosphate solutions, and detected at three ultraviolet wavelengths. Calibration curves were linear over the range 0.025-15 microg/mL for saquinavir and 0.05-15 microg/mL for the other analytes. The accuracies ranged from -6.9% to +7.6%, and the intra-assay and inter-assay precisions were <9.2 and <11.8%, respectively. Our method, covering most of the PIs and NNRTIs currently used, facilitates ready therapeutic drug monitoring in hospital laboratories.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP154598524-A	Efavirenz Ready Made Solution		154598-52-4	Price

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