Home
Resources
Publications
Simultaneous Determination of Six Toxic Alkaloids in Human Plasma and Urine Using Capillary Zone Electrophoresis Coupled to Time-Of-Flight Mass Spectrometry

Simultaneous Determination of Six Toxic Alkaloids in Human Plasma and Urine Using Capillary Zone Electrophoresis Coupled to Time-Of-Flight Mass Spectrometry

Zhuhong Yu, Zhongping Wu, Feijun Gong, Rong Wong, Chen Liang, Yurong Zhang, Yunqiu Yu

J Sep Sci. 2012 Oct;35(20):2773-80.

PMID: 22997096

Abstract:

A novel capillary zone electrophoresis separation coupled to electro spray ionization time-of-flight mass spectrometry method was developed for the simultaneous analysis of six toxic alkaloids: brucine, strychnine, atropine sulfate, anisodamine hydrobromide, scopolamine hydrobromide and anisodine hydrobromide in human plasma and urine. To obtain optimal sensitivity, a solid-phase extraction method using Oasis MCX cartridges (1 mL, 30 mg; Waters, USA) for the pretreatment of samples was used. All compounds were separated by capillary zone electrophoresis at 25 kV within 12 min in an uncoated fused-silica capillary of 75 μm id × 100 cm and were detected by time-of-flight mass spectrometry. This method was validated with regard to precision, accuracy, sensitivity, linear range, limit of detection (LOD), and limit of quantification (LOQ). In the plasma and urine samples, the linear calibration curves were obtained over the range of 0.50-100 ng/mL. The LOD and LOQ were 0.2-0.5 ng/mL and 0.5-1.0 ng/mL, respectively. The intra- and interday precision was better than 12% and 13%, respectively. Electrophoretic peaks could be identified by mass analysis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP55449495	Anisodamine hydrobromide		55449-49-5	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: