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Simultaneous Determination of Trace Levels of Ethylmercury and Methylmercury in Biological Samples and Vaccines Using Sodium Tetra(n-Propyl)borate as Derivatizing Agent

Simultaneous Determination of Trace Levels of Ethylmercury and Methylmercury in Biological Samples and Vaccines Using Sodium Tetra(n-Propyl)borate as Derivatizing Agent

Darija Gibicar, Martina Logar, Nusa Horvat, Andreja Marn-Pernat, Rafael Ponikvar, Milena Horvat

Anal Bioanal Chem. 2007 May;388(2):329-40.

PMID: 17340078

Abstract:

Because of increasing awareness of the potential neurotoxicity of even low levels of organomercury compounds, analytical techniques are required for determination of low concentrations of ethylmercury (EtHg) and methylmercury (MeHg) in biological samples. An accurate and sensitive method has been developed for simultaneous determination of methylmercury and ethylmercury in vaccines and biological samples. MeHg and EtHg were isolated by acid leaching (H2SO4-KBr-CuSO4), extraction of MeHg and EtHg bromides into an organic solvent (CH2Cl2), then back-extraction into Milli-Q water. MeHg and EtHg bromides were derivatized with sodium tetrapropylborate (NaBPr4), collected at room temperature on Tenax, separated by isothermal gas chromatography (GC), pyrolysed, and detected by cold-vapour atomic fluorescence spectrometry (CV AFS). The repeatability of results from the method was approximately 5-10% for EtHg and 5-15% for MeHg. Detection limits achieved were 0.01 ng g-1 for EtHg and MeHg in blood, saliva, and vaccines and 5 ng g-1 for EtHg and MeHg in hair. The method presented has been shown to be suitable for determination of background levels of these contaminants in biological samples and can be used in studies related to the health effects of mercury and its species in man. This work illustrates the possibility of using hair and blood as potential biomarkers of exposure to thiomersal.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP105146610	Sodium tetrapropylborate		105146-61-0	Price

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